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KMID : 0861020080230020169
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Korea Journal of Herbology
2008 Volume.23 No. 2 p.169 ~ p.178
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Anti-inflammatory Effect of Injinho-tang in RAW 264.7 Cells
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Yun Hyun-Jeong
Kim Byung-Wan
Kim Chang-Hyun
Park Sun-Dong
Lee Hyo-Seung
Heo Sook-Kyonug
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Abstract
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Objectives: Inflammation is important event in the development of vascular diseases including hypertension, atherosclerosis, and restenosis. Injinho-tang(IJHT) has been used as a traditional Korean herbal medicine since ancient times, and today it is widely used as a medication for jaundice associated with inflammation of the liver. The aim of this study was to determine whether IJHT and its components inhibit production of nitrite, an index of NO, and proinflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages.
Methods: Cytotoxic activity of IJHT and its components on RAW 264.7 cells was using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines were measured by ELISA kit. The levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were detected by western blot.
Results: IJHT and its components significantly inhibited the LPS-induced NO production and iNOS expression accompanied by an attenuation of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), IL-1β and monocyte chemoattractant protein-1 (MCP-1) formation in macrophages.
Conclusions: IJHT and its components inhibit LPS-induced inflammation via decreasing cytokines production. These results indicate that IJHT and its components have potential as an anti-inflammation and anti-artherosclerosis agent.
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KEYWORD
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Evodia officinalis DODE, anti-inflammatory activity, cytokine, adhesion molecule
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